Thy1-gcamp6s mice
Webb18 okt. 2012 · The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, … Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green fluorescent calcium indicator, GCaMP6f, in subsets of excitatory neurons in the brain and possibly other cell types and tissues.
Thy1-gcamp6s mice
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WebbWe quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for … WebbThe mouse Thy1 promoter drives expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6s), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence.
Webb24 sep. 2014 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. Dana H1, Chen TW1, Hu A1, Shields BC1, Guo C1, Looger LL1, Kim DS1, Svoboda K1 … Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green …
Webb16 apr. 2024 · Because multiple ACX areas contribute to auditory processing, we first performed WF imaging of GCaMP6s in awake mice. For unbiased identification of ACX areas, we developed an automated image segmentation algorithm based upon temporal responses. We detected known and other ACX areas. Webb1 juni 2024 · Transgenic Thy1-GCaMP6s mice were administered three doses of propofol during P7–P11 and assessed at 4 months of age. Immunostaining in the motor cortex revealed a 10–18% reduction in the density of NeuN + cells and GCaMP6 + pyramidal neurones in both L2/3 and L5 of the motor cortex after repeated propofol exposure ( Fig. …
Webb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory …
Webb6 apr. 2024 · We had previously shown that THY1 (CD90) is a tumor suppressor in nasopharyngeal carcinoma (NPC) and that its down-regulation and loss of expression are associated with tumor metastasis, yet the mechanism leading to such effects remains unknown. In this study we show that tumor invasion could be suppressed by THY1 via … harold gets his dad firedWebb16 sep. 2024 · The thick trace shows the mean for n = 6 mice. h, Change in power of the 1–3-Hz rhythm after administration of PCP, MK801 and memantine. The mean power at 1–3 Hz is shown ( n = 5 mice per... harold g. grasmickWebb1 mars 2024 · Thy1 -GCaMP6s mice (Jackson labs stock number 024275) were used in a subset of auditory brainstem response (ABR) measurements. Imaging experiments were performed on male and female offspring of Thy1 -GCaMP6 crossed with CBA/CaJ, a strain that retains good ABR thresholds through adulthood ( Zheng et al. 1999 ). harold getting shot in the eyeWebb22 juni 2024 · These differences may be expected, as the Thy1-GCaMP6s GP4.3 mice express GCaMP6s in a subset of excitatory cells, 16 and mice expressing GCaMP6s under the CaMKIIa promoter (Ai94, tetO) have GCaMP expressed in excitatory neurons, 50 while the AAV-PHP.eB-hSyn-GCaMP6s mice express GCaMP in all neurons. 51 For this reason, … harold gish obituaryWebbNational Center for Biotechnology Information harold gilbert shawnee ohioWebb21 jan. 2024 · The experiment was performed on transgenic female mice (3- to 4-months old) expressing the calcium indicator GCaMP6s driven by the Thy1 promoter (Thy1-GCaMP6s; Jackson mouse #024275, GP4.3, C57BL/6-Tg (Thy1-GCaMP6s)GP4.3Dkim/J16). harold g hamm net worthWebb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions... harold gilmore obituary