WebbProteins control all biological systems in a cell, and while many proteins perform their functions independently, the vast majority of proteins interact with others for proper biological activity. Characterizing protein–protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer ... Webb5 juni 2015 · By generating Tg mice expressing other split luciferase probes designed to detect particular interactions, it can be a general method for imaging protein-protein interactions in vivo. Since we aimed to observe apparent change in light emission along with CREB phosphorylation in current study, higher dose (64 mg/kg) of imipramine was …
A fluorescent indicator for visualizing cAMP-induced …
WebbIn the nervous system, protein phosphorylation is an essential feature of synaptic function. Although protein phosphorylation is known to be important for many synaptic processes … WebbDownload scientific diagram SDC4-PKCα controls srGAP2 phosphorylation in vivo. (A) Western blot of endogenous SDC4, PKCα and exogenous GFP-srGAP2 expression. β-actin as protein loading control. endemic inequality
Neutralization of Nogo-A enhances synaptic plasticity in the …
Webb28 juli 2024 · Protein kinases constitute a rich pool of biomarkers and therapeutic targets of tremendous diseases including cancer. However, sensing kinase activity in vivo while … Webb1 aug. 2024 · Protein phosphatase Fluorescent probe Cellular imaging Introduction Reversible protein phosphorylation is widely used in biology to regulate protein–protein … Webb12 jan. 2016 · Objectives. To design a new system for the in vivo phosphorylation of proteins in Escherichia coli using the co-expression of the α-subunit of casein kinase II (CKIIα) and a target protein, (Nanofitin) fused with a phosphorylatable tag.. Results. The level of the co-expressed CKIIα was controlled by the arabinose promoter and optimal … dr carly ernst scottsdale