NettetIntrathecally delivered orexin-A suppressed cataplexy and SOREM in orexin knockout mice. ( A and B) Typical examples of EEG delta power (0.5–4 Hz), EEG theta power (4–10 Hz), EMG integral, and hypnograms of an orexin knockout mouse during aCSF (vehicle) ( A) and orexin-A ( B) infusion with chocolate provided in the cage. Nettet1. feb. 2024 · Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter. Key words. Intrathecal; Gene …
Optimization of Intrathecal Administration in the Rat Charles River
Nettet17. okt. 1980 · The present paper describes the injection technique, the distribution of intrathecal methylene blue dye and [3H/mor- phine, and the analgesic response of mice to intrathecally injected morphine sulfate. 2. Materials and methods Male Swiss-Webster mice (20~25 g, Biolab Inc., St. Paul, MN) were used throughout these experiments. NettetIn 36 animals out of 41 (88%), the catheter was correctly placed in the intrathecal space. In 4 animals, the catheter was found to be in the spinal cord parenchyma (10%), and in 1 animal the catheter could not be located. These findings are in line with previously published data (Butt, M., Toxicol Pathol 2011 39:213). 2 MATERIALS AND METHODS permission types in windows
Intrathecal Infusion of Diosgenin during the Chronic Phase
NettetMouse Intrathecal . Mouse Intraperitoneal Rat Rat Jugular Rat Femoral. Intrathecal Rat Intraperitoneal. Available Catheters. CFS-03.22 Product of ... 9 and 11 mm for use in small mice. Catheter tip optimum for use with other small vessels (e.g. femoral, carotid). 0007700 Mouse Jugular (adjustable length) NettetMouse study supplies to improve animal health and welfare. Work with SAI to customize catheters, buttons and extension lines. We offer mouse tail vein and jvc catheters, harnesses, tethers, swivels, disposable 20 gauge 1.5" gavage needles w/ bite guard, and blood collection tools like butterfly needles & micro tubes. Nettet15. des. 2024 · Effects of the intrathecal diosgenin infusion on the increase in axons at the lesion center of mice with chronic spinal cord injury (SCI). Thirty-one days after SCI, diosgenin (final concentration in CSF; 0.1 μM) or a vehicle solution was intrathecally injected using a mouse intrathecal catheter and a mini osmotic pump for 59 days. permission to work permit