Goldenstar rt6 cdna synthesis mix

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cDNA Synthesis and Amplification Protocol using the Template ... - NEB

Weba Goldenstar™ RT6 cDNA Synthesis Mix kit (TsingKe Biotech Co., Ltd., Beijing, China) according to the manu-facture's instruction. Then, the first-strand cDNA was diluted to 80 … Web丁香通为您找到41条dna拼接需什么酶信息，包括dna拼接需什么酶报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna拼接需什么酶,上丁香通。 short curly stacked bob hairstyles

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WebAug 16, 2024 · The reverse transcription was carried out with Goldenstar RT6 cDNA Synthesis Kit (TSK302M, Tsingke, Beijing, China). An RNase-free EP tube was prepared, and gDNA remover, 10× gDNA remover Buffer, dNTP Mix, Oligo dT, Randomer, DTT, and RT6 were added with 1 μ L, respectively. WebMar 1, 2024 · RT-PCR was used to detect the silencing of the reprogramming exogenous factors. Goldenstar™ MIX (TSINGKE, Beijing, China) were used for amplifying cDNA under the following conditions: 98 °C for 2 min followed by 35 cycles of 98 °C for 10 s, 58 °C for 10 s and 72 °C for 5 s, then 72 °C for 2 min and 16 °C for holding. WebNov 6, 2024 · Total RNA was extracted using TRIzol reagent, and cDNA was synthesized by Goldenstar™ RT6 cDNA Synthesis Mix (TsingKe, Beijing, China) following the protocol recommended by the manufacturer. A total of 12 OBPs, all CSPs and 12 ORs were randomly selected for qRT-PCR analysis. To correct sample-to-sample variation, ß-actin … short curly stacked bob

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Goldenstar Rt6 Cdna Synthesis Kit BIOTECHCO Bioz

WebFeb 17, 2024 · Total RNA was extracted from normal skin and HS tissues of rat tails using TriPure Reagent (BIOFIT, Chengdu, China). Next, cDNA was synthesised from RNA using the Goldenstar RT6 cDNA Synthesis Kit Ver 2 (Tsingke Biotech Co., Beijing, China). RT-qPCR was performed in a 2xT5 Fast qPCR Mix (SYBR Green I; Tsingke Biotech Co.). WebSep 10, 2024 · Using cDNA originating from CB03234-S as a template, any intergenic region embodied in transcription unit would give a clear PCR product, whereas those failing to generate PCR fragments could constitute promoter areas delimiting different transcription units. ... China), and reverse transcribed into cDNA by using Goldenstar RT6 cDNA … short curly styles for black womenWebJan 8, 2024 · The cDNA from antennae of both sexes was synthesized using the Goldenstar ™ RT6 cDNA Synthesis Mix. One μg of total RNA from samples was used in reverse transcription in a total volume of 20 μL reaction system to obtain the first-stand cDNA. The qRT-PCR was performed on an ABI 7500 using SYBR green dye (2×T5 Fast … short curly stacked hairstyles for women

"WebDec 2, 2024 · First-strand cDNA Synthesis was performed with Goldenstar RT6 Mix (Tsingke). qRT-PCR reactions were prepared with SYBR Green according to manufacturer's recommendation (Tsingke). Sin1 primer sequences for qRT-PCR are as below: human-forward-GGTGGACACCGATTTCCCC, human-reverse … " - Goldenstar rt6 cdna synthesis mix

Goldenstar rt6 cdna synthesis mix

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WebMar 5, 2024 · The Goldenstar RT6 cDNA Synthesis Kit Ver.2 (TsingKe, China) was used following the manufacturer’s instructions. ... (Bio-Rad, USA), with a total volume of 20 μL reaction system containing 10 μL of 2 × T5 Fast qPCR Mix (SYBR Green I) (TsingKe, China), 0.8 μL of 10 μM Primer F, ... WebBed & Board 2-bedroom 1-bath Updated Bungalow. 1 hour to Tulsa, OK 50 minutes to Pioneer Woman You will be close to everything when you stay at this centrally-located …

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WebDec 1, 2024 · The Goldenstar™RT6 cDNA Synthesis Kit (TsingKe, Beijing, China) was used for the cDNA synthesis in a 10 μL reverse-transcription reaction mixture (5× … WebReadyScript ® cDNA Synthesis Mix is a sensitive and easy-to-use solution for two-step RT-PCR. This 5X concentrated mix provides all necessary components (except RNA template) for first-strand synthesis including: buffer, dNTPs, MgCl 2, primers, RNase inhibitor protein, ReadyScript reverse transcriptase and stabilizers.ReadyScript is a …

WebMay 20, 2024 · The strand cDNA was synthesized using Goldenstar RT6 cDNA synthesis kit (Tsingke, China). The expression levels of representative unigenes and selected key genes in the flavonoid biosynthesis pathway were analyzed by qRT-PCR using FQD-96A (Bioer Technology, China). Web产品中的Goldenstar TM RT6逆转录酶由改造的莫洛尼氏鼠白血病病毒逆转录酶(M-MLV RT)经体外大肠杆菌重组表达而来。野生型M-MLV RT在分子生物实验中有两个明显的缺 …

WebMar 15, 2024 · The precipitates were washed twice in 75% ethanol and centrifuged at 4 °C for 5 min. Total RNA was reverse-transcribed into cDNA using the Goldenstar RT6 cDNA Synthesis Kit Ver. 2 (TSK302M; TsingKe, Shanghai, China) based on the manufacturer’s instructions and used immediately or stored at −20 °C. Quantitative Real-Time PCR WebGoldenstar Rt6 Cdna Synthesis Kit, supplied by BIOTECHCO, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article …

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Web丁香通商铺北京擎科生物科技股份有限公司为您提供实验室仪器 / 设备、试剂、技术服务、细胞库 / 细胞培养、抗体、elisa 试剂盒、耗材等分类产品13件，全方位满足您的科研实验采购需求 sandy rhymesWebNov 25, 2024 · Reverse transcription step was carried out using Goldenstar RT6 cDNA Synthesis Kit Ver 2 (TsingKe, Chengdu, China) with random primer mix following the manufacturer’s manual. Quantitative real-time reverse transcription PCR was performed in the Bio-Rad iQ5 real-time PCR detection system with SuperReal PreMix (SYBR Green) … short curly tapered bobWebTotal RNA was extracted from the cells using Trizol reagent (Ambion, Life technologies), and 1 μg of RNA was reverse-transcribed using the Goldenstar RT6 cDNA Synthesis Mix (Takara, Kyoto, Japan) according to the manufacturer’s instructions. sandy ribeiroWebOct 18, 2024 · Total RNA from the four samples was extracted with an RNA extraction kit (TSINGKE, Beijing, China). Subsequently, cDNA was synthesized using the Goldenstar RT6 cDNA Synthesis Kit Ver.2 kit (Beijing TsingKe Biotech Co., Ltd.). qRT-PCR was performed using the CFX96TM real-time system (Bio-Rad, California, USA) and 2 × … short curly styles for older womenWebJul 12, 2024 · All RNA samples served as templates for the cDNA synthesis by the Goldenstar RT6 cDNA Synthesis Mix(TSINGKE, Beijing). The reverse transcribed products was kept at −20°C. Quantitative real-time PCR (qRT-PCR) was performed with Step One Plus real-time quantitative PCR instrument (Thermo Fisher, America) and … short curly styles for natural hairWebJun 1, 2024 · The Trelief™ RNAprep FastPure Tissue & Cell, Goldenstar™ RT6 cDNA Synthesis, and SYBR Green I (2 ×T5 Fast qPCR Mix) kits were all purchased from TsingKe Biotech Co., Ltd., Beijing, China. 2.5. Crayfish culture for challenge experiments. The A. hydrophila from our laboratory. We incubated it in a common agar medium at 28 °C for … short curly synthetic wigsWeb丁香通商铺北京擎科生物科技股份有限公司为您提供实验室仪器 / 设备、试剂、技术服务、细胞库 / 细胞培养、抗体、elisa 试剂盒、耗材等分类产品2件，全方位满足您的科研实验采购需求 sandy rice