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WebWhen to use X-ray film for imaging Western blots X-ray film is suitable for use with either radioactive or chemiluminescent detection. Exposure to light imprints an image on X-ray … WebIn each method of western blot detection, a detectable signal is generated following binding of an antibody specific for the protein of interest. In colorimetric detection ( A ), the signal is a colored precipitate. In chemiluminescence ( B ), the reaction itself emits light. WebWestern blot normalization allows you to faithfully compare changes in protein expression by establishing the baseline needed to correct against common errors such as inconsistent sample preparation, pipetting error, and uneven protein transfer. k s kids giddy-up \u0026 play